PROGRAMME

Full hands-on program of 4 days

• Knowledge of lab-scale bioreactors (stainless steel / single-use)
  i.e. identification of bioreactor parts / Bioreactor mounting / sterilizing
• Suspension cell culture in bioreactors (CHO, Sf9)
  i.e. comparison of culture conditions (ex. culture medium / infection conditions like MOI screening)
• Standard cell culture monitoring
  Sterile Sampling, Cell counting, identification of viral replication signs / transgene expression, Metabolic analysis / Cell death
• Cell infection vs transduction process
  i.e. kinetics / impacting parameters: MOI / Media / process / scale-up

Please find the programme as pdf file below.

Access to Level 2 laboratories equipped with

• 500 mL stainless steel Bioreactors (Applikon)
• 2 L single-use bioreactor (Sartorius Biostat A )
• 20 L Single-use  Rocker Bioreactor (Cytiva)
• Standard Cell culture equipment’s

MANDATORY PRE-REQUISITE:

• Applying academic or industrial (PhD students, Post-Doc, Engineers, etc..) should already have cell culture knowledge and, ideally, have already followed one of the ESACT theoretical courses.